Volume : 2, Issue : 10, OCT 2016

IN SILICO AND IN VITRO EVALUATION OF ANTI CANDIDAL ACTIVITY OF SELECTED MEDICINAL PLANTS

R. Sathish Kumar

Abstract

Candida spp. once a commensal microbe becomes clinically important pathogen since it is the causative for several types of infection, ranging from superficial infections such as vulvovaginal candidiasis, esophageal or oropharyngeal candidiasis, to life-threatening invasive disorders. Medicinal plants being our traditional mean of treatment, the phytoconstituents of the plants Acalypha indica, Aloe barbadensis, Andrographis paniculata, Argemone mexicana, Azadirachta indica and Vitex negundo in the present study had been screened using molecular docking studies. The compound mauritianin from Acalypha indica showed least G.score value of -11.87 Kcal/mol for exo-β-1,3 glucanase, therefore, the methanol extract of A. indica was quantified for the presence of glycosides using HPTLC technique which indicated the higher presence of glycosides. Moreover, the anti-candida activity of the methanol leaf extract of A. indica also observed to inhibit the growth of Candida albicans significantly. Mauritianin, a glycoside might be reasoned for its efficient activity against C. albicans. Further studies to identify and isolate the bioactive principle from the A. indica extract have to be determined.

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References

1. Anant G.S. and Jaya P.H. (2013). Traditional Phytotherapy of some medicinal plants used by Tharu and Magar communities of western Nepal, against dermatological disorders. Scientific world, 11(11), 81-89. 2. Bhakshu, L., Ratnam, K.V., and Raju, R.R.V. (2016). Anticandidal activity and phytochemical analysis of certain medicinal plants from Eastern Ghats, India. Indian J. Nat. Products and Resources, 7(1), 25-31. 3. Chambers, R.S., A.R. Walden, G.S. Brooke, J.F. Cutfield and Sullivan,P.A. (1993a). Identification of a putative active site residue in the exo-β-(1,3)-glucanase of Candida albicans. FEBS Letters, 327, 366-369. 4. Cutfield, S.M., G.J. Davies, G. Murshudov, B.F. Anderson, P.C.E. Moody, P.A Sullivan and Cutfield,J.F. (1999). The structure of the Exo-β-(1,3)-glucanase from Candida albicans in native and bound forms: relationship between a pocket and groove in family 5 glycosyl hydrolases. J. Mol. Biol., 294, 771-783. 5. Ellis, M. (2002). Invasive fungal infections: evolving challenges for diagnosis and therapeutics. J. Mol. Immunol., 38, 947-957. 6. Klunda, T., Machova, E., Cizova, A., Horak, r., Polakova, M., and Bystricky, S. (2016). Alkyl glycosides as potential anti-Candida albicans growth agents. Chemical Papers, 70(9), 1166-1170. 7. Lee, J.H., Lee, J.Y., Park, J.H., Jung, H.S., Kim, J.S., Kang, S.S., Kim, Y.S., and Han, Y. (2007). Immunoregulatory activity by daucosterol, a β-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice. Vaccine, 25(19), 3834-3840. 8. Lum, K.Y., Tay, S.T., Le, C.F., Lee, V.S., Sabri, N.H., Velayuthan, R.D., Hassan, H., and Sekaran, S.D. (2015). Activity of novel synthetic peptides against Candida albicans. Scientific reports, 5 (9657), 1-12. 9. Mackenzie, L.F., G.S. Brooke, J.F. Cutfield, P.A. Sullivan and Withers,S.G. (1997). Identification of Glu-330 as the catalytic nuclephile of Candida albicans exo-β-(1,3)-glucanase. J. Biol. Chem., 272, 361-3167.
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10. Nagalingam, M., Arumugam, G., and Panneerselvam, A., (2015). Antimicrobial activity of some Indian folklore medicinal plants against drug resistant bacteria and fungi isolated from clinical samples. Asian J. Plant Sci. Res., 5(6), 49-56. 11. Nakatani, Y., Larsen, D.S., Cutfield, S.M., and Cutfield, J.F. (2014). Major change in regiospecificity for the Exo-1,3-β- glucanase from Candida albicans following its conversion to a glycosynthase. Biochem., 53(20), 3318-3326. 12. Oliveira, S.G.D., Lund, R.G., Carvalho, R.V., Pereira, C.M.P., and Piva, E. (2016). Anti-candida and anti-enzyme and cytotoxicity of 2-phenyl-4H-chromen-4-one. Afr. J. Microbiol. Res., 10(7), 219-224. 13. Sardi, J.C., Almeida, A.M., and Mendes Giannini, M.J. (2011). New antimicrobial therapies used against fungi present in subgingival sites- a brief review. Arch. Oral Biol., 56, 951-959. 14. Sathish kumar, R., Lakshmi, P.T.V. and Annamalai, A. (2015). In silico analysis of binding efficiency of Quercetin with cancer targets. Adv. Biomed. Pharma., 2(4), 169-174. 15. Somchit, M.N., Abdul Rashid, R., Abdullah, A., Zuraini, A., Zakaria, Z.A., Sulaiman, M.R., Arifah, A.K., and Mutalib, A.R. (2010). In vitro antimicrobial activity of leaves of Acalypha indica Linn. (Euphorbiaceae). Afr. J. Microbiol. Res., 4(20), 2133-2136. 16. Suresh, M., Rath, P.K., Pannerselvan, A., Dhanasekaran, D., and Thajuddin, N. (2009). Antifungal activity of selected Indian Medicinal plant salts. J. Global Pharm. Technol., 71-74. 17. Tariq, A.L., Priya, U., and Lone, R.A. (2015). Medicinal plants Acalypha indica and Prosopis gladulosa an alternative medication for Candidiasis. Int. J. curr. Microbiol. App. Sci., 4(8), 343-351. 18. Xu, H., Nobile, C.J., and Dongari-Bagtzoglou, A. (2013). Glucanase induces filamentation of the fungal pathogen Candida albicans. PlosOne, 8(5), 1-7.